Vanilla (Vanilla planifolia Andr.) : 9783659683541 : 17 Feb 2015 : The effect of rooting media and number of nodes per cutting on nursery performance of vanilla fragrance were evaluated at south western Ethiopia in 2011/ 2012. Six rooting media (forest soil, decomposed animal manure, fine sand, 1:1 mixture of forest soil: fine sand, 1:1 mixture of decomposed animal manure: fine sand and 1:1:1 mixture of forest soil: decomposed animal manure: fine sand) and four levels of node number (two, three, four, and five node cutting) were used in this experiment. Treatments were arranged in Randomized Complete Block Design with three replications. The result revealed that interaction between the two factors was significant (P0.05) for all parameters studied except sprouting percentage and root number. The highest shoot length, shoot girth, shoot fresh weight, shoot dry weigh, leaf number, leaf area, leaf fresh weight, leaf dry weight and root to shoot ratio were obtained from four node cuttings