Chemical and Biological Studies on Some Indian Medicinal Plants : LAP Lambert Academic Publishing : 9786203196573 : 6203196576 : 23 Dec 2020 : In the present study, a novel two step CombiFlash chromatographic method wasdeveloped for the fast isolation of glycyrrhizic acid, the major bio-active marker ofG. glabra. Further, glycyrrhizic acid was quantitatively estimated in the root part ofG. glabra and two of its commercial samples such as Yastimadhu churna and Artinby employing a rapid and validated HPTLC method. The method is rapid, simple and reproducible. The marker compound, glycyrrhizic acid was found to present in 0.88% in the roots of G. glabra and 0.78% in Yastimadhu churna. But surprisingly, it could not be detected in Artin capsules. The developed method can be successfully employed for quality control and quality assurance of G. glabra, where the root part is used.