Polyhydroxybutyrate (PHB) : LAP Lambert Academic Publishing : 9786207461745 : 6207461746 : 26 Feb 2024 : Poly hydroxyl butyrate (PHB) can be used as an effective thermoplastic, and has many characteristics similar to those of standard commercial plastics like polypropylene. PHB- based plastic substitutes are less flexible than traditional plastics; they are completely biodegradable and leave behind no residue. The present study deals with the isolation, screening, production and extraction of Polyhydroxy butyrate from bacillus species. And characterization of biopolymer (PHB) producing bacteria from different soil sample in and around the Bangalore. Out of a total of 3 isolated strains one strain was selected for PHB production in different conditions like incubation temperature and time. The bacteria were screened by morphological and biochemical characteristics as Bacillus species. The bacteria were further identified by Sudan black- B staining and molecular characterization. Regardi