Insights into the assembly of NiFe hydrogenase enzymes : 9783639669756 : 09 Dec 2014 : Certain photosynthetic organisms have the capability to produce molecular hydrogen by converting solar energy into this clean energy source. Hydrogen can be burnt, high amount of clean energy can be generated, and the only byproduct is water. Certain organisms contain ancient enzymes, which can evolve hydrogen from protons and electrons. These enzymes are the hydrogenases, which catalyze the oxidation of molecular hydrogen into protons and electrons, and the reverse reaction, the reduction of protons to yield molecular H2. Considering the complexity in function and localization, in addition to the structural sophistication of [NiFe] hydrogenases and their active sites, it is not surprising that many genes are required for the biosynthesis of these enzymes. The subunits coded by the structural genes have to undergo modification processes mediated by the so-called accessory proteins. This molecular ass