iMyeloid-Derived Suppressor Cells, Volume 184i in the iMethods in Cell Biologyi series includes comprehensive and detailed protocols to assess the critical role of myeloid-derived suppressor cells MDSCs, an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume presents twelve interesting protocols that address the isolation of MDSCs in murine models, the detection of mouse and human MDSCs by flow cytometry, T-cell proliferation assays, isolation of MDSC-derived exosomes and their immunosuppressive function, characterization of lysosomal lipase in MDSCs, detection of MDSC-derived nitric oxide, and other iin vivoi and iex vivoi assays.