An efficient protocol was established for in vitro propagation of one endangered and one rare medicinal plants, Gloriosa superba Linn. and Plumbago indica L. For shoot induction in G. superba MS basal medium, supplemented with 4.0 mgl BAP 1.0 mgl IAA was required, in which 92 culture produced shoots with 4 shoots per culture, respectively, whereas for P. indica MS basal medium with 0.5 mgl BAP was needed, in which 95 culture produced shoots with 6 shoots per culture. For further development of the medium, casein hydrolysate CH 50-200mgl, activated charcoal AC 2gl and coconut water CW 5-20 were added individually to the medium increased the number of shoots upto 12 and 14 per culture. In vitro raised shoots rooted on half strength MS medium with auxin were found tobe species specific, which were 1.0 mgl each of IBA and IAA for G. superba and 0.5 mgl of IAA for P. indica. For acclimatization and transplantation, the plants in the rooting culture tubes were kept in normal room temperatur