Evaluation of Microbial Diversity in Wetlands Through Polymerase Chain Reaction and Restriction Fragment Length Polymorphism

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Evaluation of Microbial Diversity in Wetlands Through Polymerase Chain Reaction and Restriction Fragment Length Polymorphism : 9781288416790 : 01 Dec 2012 : The diversity of microbial communities in wetlands has not been fully measured. These communities may offer tools to naturally remediate sites polluted with chlorinated compounds, i.e., perchloroethylene (PCE). Restriction Fragment Length Polymorphism (RFLP) is a genomic characterization technique that is capable of rapidly characterizing bacterial communities and can provide valuable information about the diversity of microbial communities. By understanding the bacterial community researchers may be able to enhance natural attenuation of polluted sites. Soil samples were collected from a constructed wetland receiving PCEcontaminated ground water at Wright-Patterson Air Force Base (WPAFB) in Dayton Ohio and a naturally-occurring and non-polluted wetland in Beavercreek, Ohio. Samples were taken seasonally (winter, spring, summer, an

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